Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation

doi: 10.1155/2022/4571981

Figure Lengend Snippet: The primers used for RT-qPCR analysis.

Article Snippet: The specific CCR3 agonist CCL11 (420-ME-100/CF, R&D Systems, MN, USA), which was reconstituted at 100 μ g/mL in sterile PBS (Vehicle2), was injected intraperitoneally at a dose of 10 μ g/per animal 30 min before the acupuncture.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation

doi: 10.1155/2022/4571981

Figure Lengend Snippet: TN reduced eotaxin and histamine (HIS) levels and decreased the expression of CC chemokine receptor 3 (CCR3) and histamine H1 receptor (H1R) in CCD rats. The levels of eotaxin (a, b), HIS (c, d), and interleukin-6 (IL-6) (e, f) in uterus and plasma were detected by ELISA. The mRNA expression of CCR3 (g) and H1R (h) in the uterus was analyzed by RT-qPCR. (i–k) Representative blots (i), and protein expression levels of CCR3 (j) and H1R (k) in uterus. All data are expressed as the means ± SEM ( n = 6 rats/group). (b–f, j, k) One-way ANOVA followed by the Bonferroni post hoc test was used. (a, g, h) The Tamhane T2 post hoc test was used. ∗∗ P < 0.01 vs. Blank group; ∗ P < 0.05 vs. Blank group; △△ P < 0.01 vs. Model group; △ P < 0.05 vs. Model group.

Article Snippet: The specific CCR3 agonist CCL11 (420-ME-100/CF, R&D Systems, MN, USA), which was reconstituted at 100 μ g/mL in sterile PBS (Vehicle2), was injected intraperitoneally at a dose of 10 μ g/per animal 30 min before the acupuncture.

Techniques: Expressing, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation

doi: 10.1155/2022/4571981

Figure Lengend Snippet: CCR3 agonist blocked the effect of TN against CCD-induced menstrual pain. (a) The representative uterine contraction curves of all groups. The representative images of the rat uterine activities. (i–vi) Model, Model + SB328437, Model + Vehicle1, Model + CCL11 + TN, Model + Vehicle2 + TN, and Model + Vehicle1 + TN. (b) The number of contraction wave. (c) The peak-to-peak value. (d) The degree of contraction. Data were analyzed by the Tamhane T2 post hoc test and expressed as means ± SEM ( n = 6 rats/group). ∗ P < 0.05 vs. Model group; △△ P < 0.01 vs. Model + Vehicle2 + TN; △ P < 0.05 vs. Model + Vehicle2 + TN; ## P < 0.01 vs. Model + Vehicle1 + TN; # P < 0.05 vs. Model + Vehicle1 + TN.

Article Snippet: The specific CCR3 agonist CCL11 (420-ME-100/CF, R&D Systems, MN, USA), which was reconstituted at 100 μ g/mL in sterile PBS (Vehicle2), was injected intraperitoneally at a dose of 10 μ g/per animal 30 min before the acupuncture.

Techniques:

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Acupuncture Alleviates Menstrual Pain in Rat Model via Suppressing Eotaxin/CCR3 Axis to Weak EOS-MC Activation

doi: 10.1155/2022/4571981

Figure Lengend Snippet: CCR3 agonist abolished the effect of TN against CCD-induced increased ECP expression and HIS level. (a) The representative photomicrographs of ECP immunohistochemical staining of all groups in the rat uterus. (i–vi) Model, Model + SB328437, Model + Vehicle1, Model + CCL11 + TN, Model + Vehicle2 + TN, and Model + Vehicle1 + TN. (b) Histogram of IOD value of ECP in the uterus of CCD rats in each group. (c) The level of HIS in uterus was detected by ELISA. The regions are photographs at 400x magnification. The arrows indicate a positive expression of ECP. All data are expressed as the means ± SEM ( n = 6 rats/group). (b) The Tamhane T2 post hoc test was used. (c) One-way ANOVA followed by the Bonferroni post hoc test was used. ∗∗ P < 0.01 vs. Model group; △△ P < 0.01 vs. Model + Viecle2 + TN; ## P < 0.01 vs. Model + Viecle1 + TN; # P < 0.05 vs. Model + Viecle1 + TN.

Article Snippet: The specific CCR3 agonist CCL11 (420-ME-100/CF, R&D Systems, MN, USA), which was reconstituted at 100 μ g/mL in sterile PBS (Vehicle2), was injected intraperitoneally at a dose of 10 μ g/per animal 30 min before the acupuncture.

Techniques: Expressing, Immunohistochemical staining, Staining, Enzyme-linked Immunosorbent Assay